Before accepting the Michael Jackson Video Vanguard awards at the MTV VMAs, JT rocked the house with a medly of his greatest hits, new and old, and even reunited with N'Sync on stage.
And while Justin's mom Lynn was beside him as he accepted the award for Video of the Year, many wondered where his wife Jessica Biel was.
Although Jess didn't make it to the show, she did hit hit the VMA after party at PH-D at the Dream Downtown, wearing a sheer black dress.
Justin arrived separately to the bash, wearing a black and white shirt and partied inside the soiree with his N'Sync bandmates.
The "Mirrors" singer was definitely feeling festive after his big wins and Instagrammed a photo of himself drinking champagne straight from the bottle.
Also spotted at the starry after party was Jay-Z, Beyonce and Rihanna, who gave JT a shout out on Twitter.
"Justin Timberlake, congratulations!!!! And what a tremendous performance," she Tweeted.